2. Signaling Pathways
  3. Cytoskeleton
  4. Arp2/3 Complex

Arp2/3 Complex

Actin-related protein 2/3 complex

Arp2/3 Complex

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The Arp2/3 complex is originally identified in Acanthamoeba and consists of seven proteins (actin-related proteins; Arp2 and Arp3, and Arp2/3 complex subunits; ARPC1-5) that are conserved in all eukaryotes, with the exception of some algae, microsporidia and protists. The complex plays an essential role in a wide variety of cellular processes including lamellipodia-mediated cell migration, endocytosis and phagocytosis, by virtue of its ability to generate branched actin filament networks.

Activation of Arp2/3 requires interaction with actin nucleation-promoting factors (NPFs). Regulation of Arp2/3 activity is achieved by endogenous inhibitory proteins through direct binding to Arp2/3 and competition with NPFs or by binding to Arp2/3-induced actin filaments and disassembly of branched actin networks. Arp2/3 inhibition has recently garnered more attention as it has been associated with attenuation of cancer progression, neurotoxic effects during drug abuse, and pathogen invasion of host cells.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-N6682
    Cytochalasin D
    		Inhibitor 99.79%
    Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap.
    Cytochalasin D
  • HY-16928
    Cytochalasin B
    		99.84%
    Cytochalasin B is a cell-permeable mycotoxin binding to the barbed end of actin filaments, disrupting the formation of actin polymers, with Kd value of 1.4-2.2 nM for F-actin. Cytochalasin B blocks cell migration.
    Cytochalasin B
  • HY-16926
    CK-666
    		Inhibitor 99.17%
    CK-666 is a cell-permeable actin-related protein Arp2/3 complex inhibitor (IC50=12 μM). CK-666 binds to Arp2/3 complex, stabilizes the inactive state of the complex, blocking movement of the Arp2 and Arp3 subunits into the activated filament-like (short pitch) conformation.
    CK-666
  • HY-16931
    SMIFH2
    		Inhibitor 98.45%
    SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton.
    SMIFH2
  • HY-16929
    Latrunculin A
    		Inhibitor ≥98.0%
    Latrunculin A (LAT-A), found in the red sea sponge Latrunculia magnifica, is a G-actin polymerization inhibitor. Latrunculin A binds to actin monomers and inhibits polymerization of actin with Kds of 0.1, 0.4, 4.7 μM and 0.19 μM for ATP-actin, ADP-Pi-actin, ADP-actin and G-actin, respectively. Latrunculin A has effective anti-metastatic properties for cancer research. Latrunculin A blocks cell migration.
    Latrunculin A
  • HY-NP136
    Myelin basic protein (human)
    		Modulator
    Myelin basic protein human, the second most abundant protein in central nervous system myelin, is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin. Myelin basic protein human mediates interactions with actin and tubulin and effect of post-translational modifications.
    Myelin basic protein (human)
  • HY-123312
    CK-548
    		Inhibitor
    CK-548 (CK-0993548) is a Arp2/3 complex inhibitor with the IC50 of 11 μM. CK-548 inhibits HIV-1 nuclear migration and infection.
    CK-548
  • HY-127042
    6,7-Epoxy-latrunculin A
    		Inhibitor
    6,7-Epoxy-latrunculin A can be isolated from the Red Sea sponge Negombata magnifica and is initially identified as an inhibitor of actin polymerization by its morphological effects.
    6,7-Epoxy-latrunculin A
  • HY-P0027
    Jasplakinolide
    		Activator 99.50%
    Jasplakinolide is a potent actin polymerization inducer and stabilizes pre-existing actin filaments. Jasplakinolide binds to F-actin competitively with phalloidin with a Kd of 15 nM. Jasplakinolide, a naturally occurring cyclic peptide from the marine sponge, has both fungicidal and anti-cancer activity.
    Jasplakinolide
  • HY-12534
    Wiskostatin
    		Inhibitor 99.58%
    Wiskostatin is a potent and selective inhibitor of neuronal Wiskott-Aldrich syndrome protein (N-WASP)-mediated actin polymerization by stabilization of the closed, autoinhibited conformation, thereby preventing Arp2/3 complex activation. Wiskostatin is also a dynamin inhibitor with an IC50 value of 20.7 μM and a potent inhibitor of clathrin-mediated endocytosis with an IC50 value of 6.9 μM. Wiskostatin causes a rapid, profound, and irreversible decrease in cellular ATP levels. Wiskostatin also induces disassembly of podosomes in a murine monocyte cell line.
    Wiskostatin
  • HY-15892
    CK-636
    		Inhibitor 99.95%
    CK-636 is a cell permeable inhibitor of Arp2/3 complex, that could inhibit actin polymerization, with IC50 values of 4 μM, 24 μM and 32 μM for human, fission yeast and bovine, respectively. CK636 blocks cell migration.
    CK-636
  • HY-114657A
    Benproperine phosphate
    		Inhibitor 99.35%
    Benproperine phosphate is an orally active, potent actin-related protein 2/3 complex subunit 2 (ARPC2) inhibitor. Benproperine phosphate attenuates the actin polymerization rate of action polymerization nucleation by impairing Arp2/3 function. Benproperine phosphate has the potential for a cough suppressant and suppresses cancer cell migration and tumor metastasis.
    Benproperine phosphate
  • HY-P2270
    Phalloidin-TRITC
    		Chemical
    Phalloidin-TRITC is a fluorescein derivative of Phalloidin, which can specifically label myof lin and display red fluorescence when labeled and can be observed using Tesred channels.
    Phalloidin-TRITC
  • HY-18931
    (Rac)-NSC305787
    		Inhibitor 99.41%
    NSC305787 is an inhibitor of ezrin with a Kd of 5.85 μM, inhibits the phosphorylation of ezrin caused by PKCΙ with an IC50 of 8.3 μM, has antitumor activity.
    (Rac)-NSC305787
  • HY-16927
    CK-869
    		Inhibitor 99.94%
    CK-869 is an Actin-Related Protein 2/3 (ARP2/3) complex inhibitor, with an IC50 of 7 μM.
    CK-869
  • HY-18931A
    (Rac)-NSC305787 hydrochloride
    		Inhibitor 99.07%
    NSC305787 hydrochloride is an inhibitor of ezrin with a Kd of 5.85 μM, inhibits the phosphorylation of ezrin caused by PKCΙ with an IC50 of 8.3 μM, has antitumor activity.
    (Rac)-NSC305787 hydrochloride
  • HY-148683
    EG-011
    		Activator 98.77%
    EG-011 is the first-in-class and potent Wiskott-Aldrich syndrome protein (WASP) activator. EG-011 activates the auto-inhibited form of WASP with strong actin polymerization. EG-011 has selective anti-tumor activity in lymphomas.
    EG-011
  • HY-P1045
    187-1, N-WASP inhibitor
    		Inhibitor 98.04%
    187-1, N-WASP inhibitor, a 14-aa cyclic peptide, is an allosteric neural Wiskott-Aldrich syndrome protein (N-WASP) inhibitor. 187-1, N-WASP inhibitor potently inhibits actin assembly induced by phosphatidylinositol 4,5-bisphosphate (PIP2) with an IC50 of 2 μM. 187-1, N-WASP inhibitor prevents the activation of Arp2/3 complex by N-WASP by stabilizing the autoinhibited state of the protein.
    187-1, N-WASP inhibitor
  • HY-P2463
    Fequesetide
    		99.36%
    Fequesetide, a peptide segment, is the active site within the protein thymosin β4 responsible for actin binding, cell migration and wound healing.
    Fequesetide
  • HY-P2031
    Phallacidin
    Phallacidin is a member of the phallotoxin family of mushroom toxins. Phallacidin binds F-actin.
    Phallacidin
Cat. No. Product Name / Synonyms Application Reactivity